Spectrophotometer

Determination of the unknown closeness and absorbance of Cobalt Chloride solution by operating the Spectrophotometer Van Nguyen BIOSC 48, Sec 21453 Date September 11, 2012 mental hospital We are going to use spectrophotometric techniques by using a spectrophotometer, which sum of moneys the absorbance by a solution of light of specific wavelength. Also, it allows us to determine the unknown concentration of solution. To do this, we will dash the summation of solution in cuvettes that are placed in the spectrophotometer. Then, spectrophotometer will show the amount of light that is absorbed by the solutes in solution. Spectrophotometry takes advantage of the dual nature of light and however work with visible light. In this experiment, I was introduced to the spectrophotometry as gallant up as how it is used to measure the concentration of aggregate CoCl2. By seeing the absorbance of CoCl2 at different wavelengths and using the dot wavelength, you than stack use the absorbance to predict the concentration of unknown tubes. My system on the concentration of unknown 2 and unknown 4 were somewhat thirty mg/ml and around x mg/ml. (I did not do the unknown number 1, 3, and the sincerely unknown.

I took the answers from my classmate group) Method First, I determined the compactness of CoCl2 by measuring the absorbance of light at different wavelengths. Second, I constructed a standard curve for cobalt chloride; and then raise expose the equation of absorption spectrum. Third, using that equation to measure the amount of an unknown concentration of CoCl2. For this experiment, we could have seven hulking test tubes, 8 smal! l test tubes; three conical tubes containing CoCl2 solution, DI water supply, and pipettes. For large tubes, each filled with different concentration of CoCl2 (0, 0.1ml, 1.0ml, 2.0ml, 3.0ml, 4.0ml, 5.0ml) plus distilled water (10, 9.9ml, 9.0ml, 8.0ml, 7.0ml, 6.0ml, 5.0ml), and labeled form 0 to 6. The deposit-go tube was containing only if distilled water (10ml) acts as a...If you want to get a beneficial essay, order it on our website: OrderCustomPaper.com

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